Anti SARS-CoV Nucleocapsid (N) Protein

ELISA results of Rabbit Anti SARS CoV Nucleocapsid (N) Protein Variants. Each well was coated in duplicate with 0.5ug/mL of SARS CoV 2 Canonical Nc [Red Line], SARS CoV 2 Omicron Nc [Green Line], SARS CoV 2 Omicron BA.2 Nc [Blue Line]. The starting dilution of antibody was 2μg/mL and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. SARS CoV 2 Nc IC50: 5 ng/mL, SARS CoV 2 Omicron Nc IC50: 7 ng/mL, SARS CoV 2 BA.2 Nc IC50: 7 ng/mL. Assay performed using Goat Anti Rabbit IgG HRP conjugated (p/n 611 1302) and TMB substrate (p/n TMBE 1000).

Immunofluorescence of SARS-CoV-2 infection in FFPE cell pellets from in vitro cultured human lung cells infected with SARS-CoV-2. Anti-SARS-CoV-2 Nucleocapsid Protein antibody (p/n 200-401-A50) is visualized with PhenoCycler reporters in red and DAPI-stained chromatin in blue. Image provided by Akoya Biosciences.

Immunofluorescence of SARS-CoV-2 infection in FFPE cell pellets from in vitro cultured human lung cells infected with SARS-CoV-2. Costaining with Anti-SARS-CoV-2 Nucleocapsid Protein antibody (p/n 200-401-A50) in red, Anti-SARS-CoV-2 Spike S1 antibody RBD antibody (antibodies-online p/n ABIN6952546) in green, and DAPI-stained chromatin in blue. Image provided by Akoya Biosciences.

Fitting of Kinetic Data to bivalent binding site model. Steady state signals are fitted to a bivalent binding site model. A 4-parameter non-linear regression curve was used for the integration of the data. The Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein Antibody (p/n 200-401-A50) presents a KD=21nM (affinity constant) to SARS-CoV Nucleocapsid (N) Protein.

Binding mode and kinetic analysis of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein Antibody (p/n 200-401-A50). Using Octet R4, SARS COV-1N was immobilized at 20 ug/mL on an AR2G biosensor and exposed to Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein Antibody over a range of 2.0 nM ？ 16.7 uM. Each curve represents the acquired signal of its protein concentration. The interaction fits to a bivalent binding site kinetic model. The association constant (ka=5.2x10^3 M^-1 s^-1) and dissociation constant (kd=1.1x10^-4 s^-1) values are depicted in the graph. The assay was performed as per the manufacturer’s protocol.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection. A549 cells over-expressing ACE2 were either infected with the SARS-CoV-2 Washington isolate or Omicron Variant at an MOI of 0.5 for 24 hours. The cells were then fixed in 10% Formalin and stained overnight at 4C with primary antibodies directed against SARS-CoV Nucleocapsid (N) (Rockland p/n 200-401-A50) at 1:1000 dilution. Imaged using an anti-rabbit secondary conjugated to AlexaFluor 568 [red] and Nuclear Counterstain DAPI [blue]. Image Courtesy of Mohsan Saeed Lab/Da-Yuan Chen, National Emerging Infectious Diseases Laboratories (NEIDL), Boston University.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection. Vero E6 cells were either infected with the SARS-CoV-2 Washington isolate (INF) at an MOI of 0.1 or uninfected (UNI) for 24 hours. The cells were then fixed in 4% PFA and stained overnight at 4C with primary antibodies directed against SARS-CoV Nucleocapsid (N) at 1:1000 dilution. Imaged using an anti-rabbit secondary conjugated to AlexaFluor 568 [red] and Nuclear Counterstain DAPI [blue]. Image Courtesy of Mohsan Saeed Lab/Da-Yuan Chen, National Emerging Infectious Diseases Laboratories (NEIDL), Boston University.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].

Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.

Immunohistochemistry of Rabbit Anti-SARS CoV Nucleocapsid Antibody. Pre-treated tissue: (A) human lung control, (B) interferon stimulated genes (ISG) low, (C) ISG high human COVID-19 lung: interferon stimulated genes (ISGs). [One pattern showes high expression of interferon stimulated genes (ISGs) and cytokines, high viral loads and limited pulmonary damage, the other pattern showes severely damaged lungs, low ISGs, low viral loads and abundant immune infiltrates.] Primary Antibody: Anti-SARS-CoV at 1:6400. Staining and Detection with Bond Polymer Refine Red Detection. [Courtesy of Mertz K. Profiles of COVID-19 lungs.]