Anti SARS-CoV Nucleocapsid (N) Protein  

Immunofluorescence of Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody. Tissue: human Liver ductal organoids. Fixation: 4% PFA. Permeabilization: 0.25% Triton X-100. Antigen retrieval: not required. Primary antibody: Rabbit Anti-SARS-CoV (N) Antibody and Mouse Anti-E-Cadhedrin Antibody at 1:500 overnight at 2-8°C. Secondary antibody: Donkey Anti-Rabbit IgG CY3 Conjugated; Donkey Anti-Mouse IgG AlexaFluor 488 Conjugated for 1hr at RT. Nuclear Counterstain: DAPI. Staining showing Mock and Infected tissue: SARS-CoV Red signal, E-Cadhedrin green signal, with DAPI (blue) nuclear counterstain. [Zhao et al. (2020)]

Immunofluorescence of Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody. Tissue: human Liver ductal organoids. Fixation: 4% PFA. Permeabilization: 0.25% Triton X-100. Antigen retrieval: not required. Primary antibody: Rabbit Anti-SARS-CoV (N) Antibody and Mouse Anti-E-Cadhedrin Antibody at 1:500 overnight at 2-8°C. Secondary antibody: Donkey Anti-Rabbit IgG CY3 Conjugated; Donkey Anti-Mouse IgG AlexaFluor 488 Conjugated for 1hr at RT. Nuclear Counterstain: DAPI. Staining of Infected and Merged: SARS-CoV Red signal, E-Cadhedrin green signal, with DAPI (blue) nuclear counterstain. [Zhao et al. (2020)]

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].

Immunofluorescence of Rabbit Anti-SARS CoV (M) Protein Antibody. Cells: Caco-2 cells 24 hours post-infection. Fixation: 4% PFA. Permeabilization: 0.3% Triton X-100. Blocking: 5% fetal calf serum/PBS. Primary Antibody: Anti-SARS-CoV nucleocapsid (N) protein at 1:1000 for 1 hour at RT. Staining: N-Protein [Red], F-actin [White], DAPI, [Blue]. Imaged: Zeiss LSM800 microscope. [Images Courtesy of AG Robert Grosse, Institute of Experimental and Clinical Pharmacology I, University of Freiburg/Svenja Ulferts and AG Georg Kochs Labs/Sebastian Weigang]. [Bouhaddou M et al. 2020]

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection. Vero E6 cells were either infected with the SARS-CoV-2 Washington isolate (INF) at an MOI of 0.1 or uninfected (UNI) for 24 hours. The cells were then fixed in 4% PFA and stained overnight at 4oC with primary antibodies directed against SARS-CoV Nucleocapsid (N) at 1:1000 dilution. Imaged using an anti-rabbit secondary conjugated to AlexaFluor 568 [red] and Nuclear Counterstain DAPI [blue]. Image Courtesy of Mohsan Saeed Lab/Da-Yuan Chen, National Emerging Infectious Diseases Laboratories (NEIDL), Boston University.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection. A549 cells over-expressing ACE2 were either infected with the SARS-CoV-2 Washington isolate or Omicron Variant at an MOI of 0.5 for 24 hours. The cells were then fixed in 10% Formalin and stained overnight at 4C with primary antibodies directed against SARS-CoV Nucleocapsid (N) (Rockland p/n 200-401-A50) at 1:1000 dilution. Imaged using an anti-rabbit secondary conjugated to AlexaFluor 568 [red] and Nuclear Counterstain DAPI [blue]. Image Courtesy of Mohsan Saeed Lab/Da-Yuan Chen, National Emerging Infectious Diseases Laboratories (NEIDL), Boston University.

Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.

Immunohistochemistry of Rabbit Anti-SARS CoV Nucleocapsid Antibody. Pre-treated tissue: (A) human lung control, (B) interferon stimulated genes (ISG) low, (C) ISG high human COVID-19 lung: interferon stimulated genes (ISGs). [One pattern showes high expression of interferon stimulated genes (ISGs) and cytokines, high viral loads and limited pulmonary damage, the other pattern showes severely damaged lungs, low ISGs, low viral loads and abundant immune infiltrates.] Primary Antibody: Anti-SARS-CoV at 1:6400. Staining and Detection with Bond Polymer Refine Red Detection. [Courtesy of Mertz K. Profiles of COVID-19 lungs.]

Western Blot of Rabbit Anti-SARS CoV Nucleocapsid (N) Protein Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: SARS CoV Nucleocapsid (N) Protein [20ng]. Primary Antibody: Anti-SARS CoV Nucleocapsid (N) Protein Antibody at 1.0μg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-1302) at 1:40000 for 30mins at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~46kDa. Observed MW: ~48kDa.

Western blot using Rockland's Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46-kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDye？800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:10,000. The fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.