Anti Rreb1  

Western blot using Rockland's Protein A Purified anti-RREB1 antibody shows detection of a predominant band believed to be RREB1 in various cell lysates (1 - HEK293, 2 - RFP-RREB transfected HEK293, 3 - M460 and 4 - T1165). All lysates were loaded at 20 μg per lane and separated by SDS-PAGE. After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1,000. The membrane was washed and reacted with IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122). IRDye800 fluorescence image was captured using the Odyssey^(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication, Shuling Zhang, CCR, NCI, Bethesda, MD.

Protein complexes associated with CtBP1 mutants. (A and B) HeLa cells were transfected with Flag-CtBP1 (Fg-CtBP1) or mutants and immunoprecipitated (IP) with the Flag Ab, and the Western blots (W) were probed with the indicated Abs. Flag-CtBPs were detected by chemiluminescence. (C) The interaction of core constituents with CtBP1. HeLa cells were transfected with Flag-CtBP1 or T7-CtBP1 and increasing concentrations (1, 5, and 10 μg) of Myc-HDAC2, Myc-CoREST, or Flag-LSD1, immunoprecipitated, and analyzed by Western blotting with the indicated Abs. (D) GST pull-down assays. Bacterially expressed His(6)-CtBP1 was incubated with GST, GST-HDAC2, or GST-CoREST, and the bound fractions were analyzed by Western blotting using CtBP1 Ab. Baculovirus-expressed Flag-LSD1 was incubated with GST or GST-CtBP1, and the bound and unbound fractions were analyzed by Western blotting using the Flag Ab. Fig 3. PMID:？17967884