| 別品名 |
GAP modifying protein 1 antibody, GMP 1 antibody, GMP1 antibody, PIC 1 antibody, PIC1 antibody, SENP2 antibody, Sentrin 1 antibody, Sentrin antibody, Small ubiquitin related modifier 1 antibody
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Ion Exchange /Gel Filtration
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| 適用 |
Western Blot
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 交差種 |
Human
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| GENE ID |
7341
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| Accession No.(Gene/Protein) |
NP_001005781, P63165
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| Gene Symbol |
SMT3
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| 形状 |
凍結乾燥品
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| 参考文献 |
[Pub Med ID]25756623
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin 1/F box protein) /CBC(cullin 2/elongin B/elonginC) like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head to tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
Western blot of hSUMO fusion protein. Anti-SUMO antibody, generated by immunization with recombinant human SUMO, was tested by western blot against a SUMO-GFP fusion protein after cleavage by proteases. Dilution of the antibody between 1:1,000 and 1:5,000 showed strong reactivity specifically with the SUMO portion of the fusion protein ~11.5kDa (arrowhead). In this blot the antibody was used at a 1:2000 dilution incubated overnight at 4 C in 5% BLOTTO (p/n B501-0500) in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (p/n 611-703-127) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication.
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Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin 1/F box protein) /CBC(cullin 2/elongin B/elonginC) like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head to tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
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| メーカー |
品番 |
包装 |
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RKL
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200-401-441
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500 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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