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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot showing detection of Maltose Binding Protein (MBP). Lane 1: MW markers. Lane 2: Maltose Binding Protein (p/n 000-001-385) [0.05 μg]. Protein was run on a 4-20% gel and transferred to 0.45 μm nitrocellulose. Blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. Primary Antibody: Anti-MBP (RABBIT) antibody (p/n 200-401-385) was used at 1:1000 overnight at 4°C. Secondary Antibody: Anti-Rabbit IgG (GOAT) IRDye800(R) conjugated antibody (p/n 611-131-002) was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 30 min at 20°C. Imaged on the LiCor Odyssey imaging system. Predicted MW: ~42 kDa, the other bands present are recombinant MBP breakdown.
Western Blot of Anti-MBP epitope tag polyclonal antibody. Lane 1: 1.0 ug of recombinant protein containing the MBP epitope tag (p/n 000-001-385). Primary Antibody: Polyclonal rabbit-anti-MBP epitope tag at 0.5-1.0 ug/ml for 1 h at room temperature. Secondary Antibody: 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. Imaging: LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Predicted MW: ~42 kDa. A minor band at corresponding to multimers of this protein is also evident.
Pull-down assays were performed to determine the effects of phosphorylation on interactions between the ICDs of ACR4 and the CRRs. The autophosphorylation status of each protein was varied for individual experiments, (+) denotes autophosphorylated protein, (-) denotes unphosphorylated protein. In (A), mACR4 (-) and sCRRs (-), (B) mACR4 (+) and sCRRs (+), (C) mACR4 (+) and sCRRs (-), (D) mACR4 (-) and sCRRs (+). In A-D, proteins were separated by 12% SDS-PAGE (top panels) and the presence of interacting proteins was determined by anti-SUMO (p/n 200-401-428) western blot (lower panels). In the bottom panel of A, anti-MBP (p/n 200-401-385) western blot demonstrates the presence of mACR4 in each reaction. Fig 2.？ PMID:？25756623
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Western Blot showing detection of Maltose Binding Protein (MBP). Lane 1: MW markers. Lane 2: Maltose Binding Protein (p/n 000-001-385) [0.05 μg]. Protein was run on a 4-20% gel and transferred to 0.45 μm nitrocellulose. Blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. Primary Antibody: Anti-MBP (RABBIT) antibody (p/n 200-401-385) was used at 1:1000 overnight at 4°C. Secondary Antibody: Anti-Rabbit IgG (GOAT) IRDye800(R) conjugated antibody (p/n 611-131-002) was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 30 min at 20°C. Imaged on the LiCor Odyssey imaging system. Predicted MW: ~42 kDa, the other bands present are recombinant MBP breakdown.
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| 別品名 |
rabbit anti-MBP Epitope Tag Antibody, rabbit anti-Maltose Binding Protein Antibody
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Purified
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| Tag情報 |
MBP
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| 参考文献 |
[[Cosmo Reference Collection]][Pub Med ID]36807264,29358299,30089259,27182662,25756623
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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200-401-385
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1 MG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
毒
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| 保存温度 |
４℃
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