Anti Maltose Binding Protein (MBP) Epitope Tag

Western Blot of Anti MBP epitope tag polyclonal antibody. Lane 1: 1.0 ug of recombinant protein containing the MBP epitope tag (p/n 000 001 385). Primary Antibody: Polyclonal rabbit anti MBP epitope tag at 0.5 1.0 ug/ml for 1 h at room temperature. Secondary Antibody: 1:2500 dilution of IRDye 800 conjugated Gt a Rabbit IgG [H&L] (code 611 132 122) for 30 min at room temperature. Imaging: LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Predicted MW: ~42 kDa. A minor band at corresponding to multimers of this protein is also evident.

Anti-MBP epitope tag polyclonal antibody detects MBP-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-MBP epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the MBP epitope tag. The apparent molecular weight of this band is 42 kDa. A minor band at corresponding to multimers of this protein is also evident. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Anti-MBP epitope tag polyclonal antibody detects MBP-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-MBP epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the MBP epitope tag. The apparent molecular weight of this band is 42 kDa. A minor band at corresponding to multimers of this protein is also evident. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Western Blot showing detection of Maltose Binding Protein (MBP). Lane 1: MW markers. Lane 2: Maltose Binding Protein (p/n 000-001-385) [0.05 ug]. Protein was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. Blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20C. Primary Antibody: Anti-MBP (RABBIT) antibody (p/n 200-401-385) was used at 1:1000 overnight at 4C. Secondary Antibody: Anti-Rabbit IgG (GOAT) IRDye800R conjugated antibody (p/n 611-131-002) was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 30 min at 20C. Imaged on the LiCor Odyssey imaging system. Predicted MW: ~42 kDa, the other bands present are recombinant MBP breakdown.