Anti Mesothelin

Flow Cytometry Results of Anti Mesothelin (MOUSE) Monoclonal Antibody. The green histogram shows NCI H226 cells and blue histogram shows MCF 7 cells. Both cell lines are stained with a 1:200 dilution Anti Mesothelin (MOUSE) Monoclonal Antibody. The secondary antibody use was Anti Mouse IgG (H&L) (GOAT) Antibody DyLightTM 488 Conjugated (p/n 610 141 002, lot#43322) at the 1:400 dilution.

Immunohistochemistry using Rockland's anti-mesothelin antibody to react with two epitopes on mesothelin in PEFF human mesothelioma tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used at 10 μg/mL to label these sections as follows: C, MAb MB; and D, MAb MN followed by goat anti-mouse IgG conjugated to horseradish peroxidase at 25 μg/mL in 1% BSA/PBS for 30 minutes. (magnification, ×200; bar, 50 μm). Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.

SDS-PAGE of Mouse anti-Mesothelin Monoclonal Antibody. Lane 1: Non-Reduced Mouse anti-Mesothelin Monoclonal Antibody. Lane M: 3 uL OPAL Pre-stained Marker (p/n MB-210-0500). Lane 2: Reduced Mouse anti-Mesothelin Monoclonal Antibody. Load: 1 ug per lane. Predicted/Observed size: Non-reduced at 160 kDa; Reduced at 55, 25 kDa.

Western blotting using Rockland's anti-mesothelin antibody. Load: Mesothelin-Fc (lane 1, 100 ng; lane 2, 25 ng; lane 3, 6 ng; lane 4, 2 ng; and lane 5, 0.4 ng) and CD25-Fc (lane 6, 50 ng) Primary antibody: anti-mesothelin at 1mg/ml. Secondary Antibody: ALP goat anti-mouse IgG and BCIP/NBT substrate. Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.