Anti Hsc70 (Hsp73)

Adipocyte TonEBP expression is elevated in obesity and TonEBP deficient mice resist obesity. aTonEBP mRNA levels in iWAT, eWAT, BAT, muscle, and liver from C57BL/6？J mice fed with CD (chow diet, n？=？5) or HFD (high fat diet, n？=？7) for 16 weeks. b Immunoblots (top) and quantification of protein levels (bottom) of TonEBP and Hsc70 in iWAT and eWAT (CD, n？=？4; HFD, n？=？6). c Correlation of TONEBP mRNA levels in human subcutaneous adipocytes and BMI (n？=？15). TonEBP mRNA (d) and representative immunoblots (e, top) and quantification of protein levels (e, bottom) in 3T3 L1 adipocytes transfected with miR negative control (NC), miR 30b (30b), or miR 30c (30c) (n？=？4). fiTonEBP+/Δ mice (？+？/Δ) and their TonEBP+/+ littermates (+/+) were fed with CD or HFD as indicated. f Changes in body weight after a switch to HFD (CD+/+, n？=？7; CD？+？/Δ, n？=？7; HFD+/+, n？=？13; HFD+/Δ, n？=？11). g Representative images of animals fed with HFD. h Body weight, height, fat mass, and lean mass (n？=？8). i Representative images (top) and weight (bottom) of fat pads from HFD fed animals (n？=？4). n represents number of biologically independent samples (ac, i) or animals (fh) or independent experiments with triplicate (d, e). All data are presented as mean？+？s.e.m. (a, b, f, h, i) or？+？s.d. (d, e). AU, arbitrary unit. The p values are determined by unpaired t test (a,b, h, i) or one way ANOVA (df). *p？<？0.05 vs. CD (a), NC (d), or +/+？(f, h, i). Source data are provided as a Source Data file Figure provided by CiteAb. Source: Nat Commun, PMID: 31387996.