Anti ATM, phospho Ser1981  

Anti ATM monoclonal Antibody was used to show constitutive activation of the ATM pathway in human urinary bladder cancer. Anti-ATM Protein Kinase pS1981 (clone # 7C10D8 ) was used to stain normal uroepithelium, early superficial lesions (Ta), and earliest invasive (T1) primary carcinomas. ATM protein is ubiquitously expressed, but Ser 1981-phosphorylated ATM (pS-ATM) is detectable only in tumor tissues. See Bartkova et al. for additional details. Reprinted by permission from Nature (Cell Cycle 4;(6), 2005) Macmillan Publishers Ltd.

ATM authophosphorylation.

Determination of ATM and ATMp antibodies specificity by western blotting. Western blot analysis of neuroretinas and brains from adult Atm-deficient and adult C57BL/6J mice was performed with monoclonal antibodies raised against ATM and ATMp, and with polyclonal antibody β-actin (internal control). Specific bands for ATM (350？kDa), ATMp (350？kDa), and β-actin (35？kDa) were detected by western blot analysis of protein homogenates extracted from different mouse tissues (neuroretina and brain). No band could be detected in protein homogenates from Atm-deficient mice tissues blotted with either specific ATM or specific ATMp antibody. Figure provided by CiteAb. Source: Mol Vis, PMID: 19234633.