| 別品名 |
Mitotic arrest deficient yeast homolog like 1 antibody, Mitotic spindle assembly checkpoint protein antibody
|
| 種由来 |
Human
|
| 標識物 |
Unlabeled
|
| 精製度 |
Ig fraction - Protein A
|
| 適用 |
Western Blot
Immunoprecipitation
|
| 免疫動物 |
Mouse
|
| 抗体クラス |
IgG1
|
| クローン |
17D10
|
| 交差種 |
Human
|
| GENE ID |
4085
|
| Accession No.(Gene/Protein) |
Q13257
|
| Gene Symbol |
MAD2L1
|
| 形状 |
滅菌済み液状品
|
| 参考文献 |
Chan GK, Liu ST, Yen TJ. (2005) Kinetochore structure and function. Trends Cell Biol. Oct 6; [Epub ahead of print]. Sudakin V, Yen TJ. (2004) Purification of the mitotic checkpoint complex, an inhibitor of the APC/C from HeLa cells. Methods Mol Biol.;281:199-212. Joseph J, Liu ST, Jablonski SA, Yen TJ, Dasso M. (2004) The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo. Curr Biol. 14(7):611-7.
|
| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
|
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot using Rockland's Mab anti-MAD2L1 antibody shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (p/n W09-000-364). Approximately 75 μg of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5,000 dilution of IRDye？800 conjugated Sh-a-Mouse IgG [H&L] (p/n 610-632-002) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye？800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
|
|
|
|
Western blot using Rockland's Mab anti-MAD2L1 antibody shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (p/n W09-000-364). Approximately 75 μg of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5,000 dilution of IRDye？800 conjugated Sh-a-Mouse IgG [H&L] (p/n 610-632-002) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye？800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
|
|
|
| メーカー |
品番 |
包装 |
|
RKL
|
200-301-461
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
|
|
