Anti Rhodamine  

Dot Blot of Mouse anti-Rhodamine Monoclonal Antibody. Antigen: Row 1 - Rhodamine Conjugated Streptavidin. Row 2 - Streptavidin. Load: Column 1 - 100ng. Column 2 - 33.3ng. Column 3 - 11.1ng. Column 4 - 3.70ng. Column 5 - 1.23ng. Primary antibody: Mouse anti-Rhodamine at 1:1,000 for 60 min at RT. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 1 HR at RT.

Rhodamine core structure. TRITC (tetramethylrhodamine-5-(and-6)-isothiocyanate) is the base tetramethylrhodamine molecule functionalized with an isothiocyanate reactive group (？N=C=S) at one of two hydrogen atoms on the bottom ring of the structure. This derivative is reactive towards primary amine groups on proteins, peptides and other biomolecules. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.