| 別品名 |
Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody, DNA Synthesis Inhibitor antibody, MDA 6 antibody, MDA6 antibody, Melanoma Differentiation Associated Protein 6 antibody
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
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| 免疫動物 |
Mouse
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| 抗体クラス |
IgG1κ
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| クローン |
WA-1
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| 交差種 |
Human
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| GENE ID |
1026
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| Accession No.(Gene/Protein) |
NP_510867, P38936
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| Gene Symbol |
WAF1
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Yeh, C. H. and Shatkin, A.J. (1995). A cis-acting element in Rous sarcoma virus long terminal repeat required for promoter repression by Hela nuclear protein p21. J. Biol. Chem. 270:15815-15820 Joshi, U. S. et al. (1998). Inhibition of tumor cell growth by p21WAF1 adenoviral gene transfer in lung cancer. Cancer Gene Ther. 5: 183-191. Yang, Z.Y. et al. (1995) The p21 cyclin-dependent kinase inhibitor suppresses tumorigenicity in vivo. Nat Med 1(10):1052-1056 Kagawa, S. et al. (1997) p53 expression overcomes p21WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells. Oncogene 15(16):1903-1909. Waldman, et al. (1995) p21 is necessary for the p53-mediated G1 arrest in human cancer cells. Cancer Res 55(22):5187-5190. Kovalic, J. et al., (1996) Int.J.Oncol. 9, suppl., 835. Stephane Supiot, Richard P. Hill and Robert G. Bristow (2008) Nutlin-3 radiosensitizes hypoxic prostate cancer cells independent of p53. Mol Cancer Ther 7; 993.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Mab anti Human p21WAF1 antibody (clone WA 1) is shown to detect human p21 by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate (p/n W09 000 364) is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti Human p21WAF1 at room temperature for 30 min followed by detection using IRDyeTM800 labeled Goat a Mouse IgG [H&L] (p/n 610 132 121) diluted 1:5,000. A single band corresponding to human p21WAF1 is detected at ~21 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p21WAF1. IRDyeTM800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI COR. IRDye is a trademark of LI COR, Inc. Other detection systems will yield similar results.
Immunofluorescence of Mouse anti-p21 antibody. Tissue: human brain. Fixation: free-floating. Antigen retrieval: not required. Primary antibody: anti-human-p21 antibody at 1:250 for 1 h at RT. Co-stained with YFP and Sox2 antibodies. Localization: p21 is nuclear and cytoplasmic. Staining: p21 as precipitated blue with Cy5, YFP as precipitated green with Cy2, and Sox2 as precipitated red with Cy3. z‐stacks from confocal expressed as one composite focal plain.
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Mab anti Human p21WAF1 antibody (clone WA 1) is shown to detect human p21 by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate (p/n W09 000 364) is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti Human p21WAF1 at room temperature for 30 min followed by detection using IRDyeTM800 labeled Goat a Mouse IgG [H&L] (p/n 610 132 121) diluted 1:5,000. A single band corresponding to human p21WAF1 is detected at ~21 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p21WAF1. IRDyeTM800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI COR. IRDye is a trademark of LI COR, Inc. Other detection systems will yield similar results.
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| メーカー |
品番 |
包装 |
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RKL
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200-301-171
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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