Anti Transferrin

Rockland primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (1 109 4134, 2 611 743 127 green); Alpha 1 anti trypsin (1 100 101 147, 2 605 742 125 red); and Human IgG (1 109 3102, 2 610 741 124 Blue) in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB 070) and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000 in MB 070 and incubated ~ 1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Biorad VersaDoc4000.

Rockland Rabbit anti-Transferrin (p/n 109-4134, lot 3033, green), Goat-anti-Alpha-1-Anti-Trypsin (p/n 100-101-147, lot 5842, red), and Mouse-a-GST (p/n 200-301-200, lot 24882, blue) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution in (p/n MB-070) overnight at 4C. Detection shown was using DyLight549 Donkey anti-Rabbit IgG (p/n 611-742-127, lot 21100),？ DyLight 488 Donkey anti-Mouse IgG (p/n 610-741-124, lot 21095), and DyLight 649 Donkey anti-Goat IgG (p/n 605-743-125, lot 20834) at 1:10000 in (p/n MB-070) 30 min RT. Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.