種由来 |
Streptomyces avidinii
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由来詳細 |
[Species]Streptomyces avidinii
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標識物 |
Unlabeled
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精製度 |
Serum
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Dot Blot
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免疫動物 |
Rabbit
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交差種 |
Streptomyces avidinii
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形状 |
凍結乾燥品
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参考文献 |
[DOI]10.1101/2022.10.31.514578
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Rockland Rabbit anti Streptavidin (200 4195 lot 23495) and Biotin conjugated Rabbit anti trypsin inhibitor antibody (200 4679 lot 6594) were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit (611 743 127 lot 20831 1:10K 1.5 hr RT in MB 070) and imaged on the BioRad VersaDoc System
Dot Blot showing the detection of Streptavidin. A three-fold serial dilution of DyLight? 488 Conjugated Streptavidin starting at 200ng was spotted onto 0.45 μm nitrocellulose and blocked in 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. Anti-Streptavidin (RABBIT) Antibody (p/n 100-4195) was incubated in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:1,000 for 1 Hour at 20°C. An HRP Gt-a-Rb secondary antibody (p/n 611-103-122) was incubated at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDocR 4000 MP.
Western Blot of Rabbit anti-Streptavidin Antibody. Lane 1: Streptavidin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Streptavidin Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 13.8 kDa, 13.8 kDa. Streptavidin exhibits a homotetramer structure with four essentially identical polypeptide chains. Pictured is reduced protein.
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Rockland Rabbit anti Streptavidin (200 4195 lot 23495) and Biotin conjugated Rabbit anti trypsin inhibitor antibody (200 4679 lot 6594) were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit (611 743 127 lot 20831 1:10K 1.5 hr RT in MB 070) and imaged on the BioRad VersaDoc System
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メーカー |
品番 |
包装 |
RKL
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100-4195
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2 ML
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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