Anti IKB α  

Western Blot of Rabbit anti-IKBα C-terminal antibody. Lane 1: IKBα C-terminal. Load: 50 ng per lane. Primary antibody: IKBα C-terminal antibody at 1:500 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min RT. Predicted/Observed size: 61 kDa, 61 kDa for IKBα C-terminal.

2-AP inhibits TNF-α/Ifn-γ-induced IκBβ degradation. Western analyses of equal amounts of protein from PC12 cell cytoplasmic extracts using polyclonal antibodies specific for IκBα or IκBβ are shown. The time and treatment are indicated above each lane？(UT？is untreated). TNF-α, Ifn-γ, and 2-AP concentrations were 10 ng/ml, 100 units/ml, and 10 mm, respectively. As a control, cells not treated with cytokine were incubated with 2-AP for 1 h (lane 2) or 3.5 h (lane 3). Where indicated, cells treated with cytokine were preincubated with 2-AP for 30 min (lanes 5,？7,9,？11,？13, and？15). Arrows？indicate each specific IκB protein. Figure 2. PMID:？9988719

EGFR induction is decreased in the presence of a constitutively active IκBα. LMP1 or CD40 was transfected into C33A cells in both the absence and the presence of a constitutively active IκBα(SS32/36AA). The resulting cell lines were analyzed by immunoblotting for EGFR expression (top) and the constitutively active IκBα(SS32/36AA) (bottom). Migration of molecular mass standards (in kilodaltons) is shown. Fig 3. PMID:？9566902