Anti Catalase  

Western Blot of Rabbit Anti-Catalase (Bovine Liver) Antibody. Lane 1: CHO K1 Lysate 10μg. Lane 2: CHO S Lysate 10μg. Lane 3: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Primary Antibody: Rabbit Anti-Catalase at 1:500 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG CY5 (p/n 611-110-122) at 1:10,000 for 30 in at RT. Expect: ~59.8kDa.

Western Blot of Rabbit anti-Catalase antibody. Lane R: Reduced samples of purified Catalase. Lane NR: Non-reduced samples of purified Catalase. Load: ~1ug of protein per lane. Primary antibody: Catalase antibody at 1:1000 for overnight at 4°C. Secondary antibody: Dylight488？ rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 59.9 kDa, 55 kDa for catalase. Other band(s): none.