別品名 |
beta-Galactosidase, beta-galactosidase, beta-gal
|
種由来 |
Escherichia coli
|
標識物 |
Unlabeled
|
精製度 |
Serum
|
適用 |
Western Blot
|
免疫動物 |
Rabbit
|
GENE ID |
12934192
|
Accession No.(Gene/Protein) |
NP_414878, P00722
|
Gene Symbol |
LacZ
|
Tag情報 |
b-GAL
|
形状 |
凍結乾燥品
|
参考文献 |
[Pub Med ID]26912623, 29017029, 29643250, 15602772, 12668621, 21890648, 27867035, 30651296, 32806578
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blotting using Rockland's Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead).?? Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate.? Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting (p/n MB-070).? The membrane was probed with fluorescein conjugated anti-b-Galactosidase (p/n 200-4236) diluted to 1:10,000.? Reaction occurred for 2 hours at room temperature.? Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDocR Imaging System developed by BIO-RAD. Other detection systems will yield similar results.
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|
Western blotting using Rockland's Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead).?? Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate.? Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting (p/n MB-070).? The membrane was probed with fluorescein conjugated anti-b-Galactosidase (p/n 200-4236) diluted to 1:10,000.? Reaction occurred for 2 hours at room temperature.? Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDocR Imaging System developed by BIO-RAD. Other detection systems will yield similar results.
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メーカー |
品番 |
包装 |
RKL
|
100-4136
|
2 ML
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
法規制 |
毒
|
保存温度 |
4℃
|
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