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Western blotting using Rockland's Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead).？？ Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate.？ Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting (p/n MB-070).？ The membrane was probed with fluorescein conjugated anti-b-Galactosidase (p/n 200-4236) diluted to 1:10,000.？ Reaction occurred for 2 hours at room temperature.？ Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc(R) Imaging System developed by BIO-RAD. Other detection systems will yield similar results.
(G) MNCs isolated from TOPGAL mice were cultured for 7 d in base medium and base medium + CHIR99021 and/or insulin as indicated. LSK Flk2−？cells were sorted from these cultures and stained for β-galactosidase (β-Gal). Cells were sorted onto lysine-coated slides, fixed with methanol, and stained using 1:50 dilution, anti-β-galactosidase (p/n 100-4136). Fig 6. PMID:？21890648.
Large GFP-LC3+？structures (clusters) form in neurons that harbor the HSV-1 genome. (a) GFP-LC3 neurons infected with KOS-58 and stained for β-Gal (red) and VP16 (white). Green arrows indicate neurons with clusters, β-Gal expression, and no VP16. White arrows indicate neurons with VP16 expression and no clusters. Bar = 50 μm. (b) Quantification of cultures described for panel a and analyzed as for？Fig. 1d. (c) Quantification of β-Gal and VP16 prevalence among total neurons (left graph) or among cluster-positive neurons (right graph) in the cultures represented in panel a. A total of ？3 experiments were performed, with >1,000 neurons scored per experiment. Error bars represent SEMs. Significance was evaluated by one-way analysis of variance (ANOVA) with Bonferroni posttests. *,？P？< 0.05. Fig 2. PMID:？26912623.
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Western blotting using Rockland's Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead).？？ Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate.？ Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting (p/n MB-070).？ The membrane was probed with fluorescein conjugated anti-b-Galactosidase (p/n 200-4236) diluted to 1:10,000.？ Reaction occurred for 2 hours at room temperature.？ Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc(R) Imaging System developed by BIO-RAD. Other detection systems will yield similar results.
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| 別品名 |
rabbit anti-BETA GALACTOSIDASE Antibody, beta-galactosidase, rabbit anti-beta-gal antibody
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| 適用 |
Western Blot
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Serum
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| Accession No.(Gene/Protein) |
NP_414878.1, P00722
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| Gene Symbol |
LacZ
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| Tag情報 |
b-GAL
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| 参考文献 |
[[Cosmo Reference Collection]][Pub Med ID]26912623,29017029,29643250,15602772,12668621,21890648,27867035,30651296,32806578
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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100-4136
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2 ML
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
毒
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| 保存温度 |
４℃
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