別品名 |
AGS2 antibody, hN2 antibody, Notch homolog 2 antibody, Notch2 antibody
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抗原部位 |
N-terminus
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Serum
|
適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry
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免疫動物 |
Rabbit
|
交差種 |
Human
|
GENE ID |
4853
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Accession No.(Gene/Protein) |
Q04721
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Gene Symbol |
NOTCH2
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]18621050, 32235870
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Rockland's anti NOTCH 2 antibody was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Western Blot of Rabbit Anti-Notch 2 N-Term Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HeLa Whole Cell Lysate (p/n W09-000-364). Lane 3: MOLT-4 Whole Cell Lysate (p/n W09-001-GK4). Lane 4: K-562 Whole Cell Lysate (p/n W09-001-GJ7). Lane 5: Human Kidney Lysate. Load: 20μg lysate/lane. Primary Antibody: Anti-NOTCH 2 at 1:1000 for 1 hr at RT. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 30mins at RT. Blocking: 5% BLOTTO. Expected MW: ~86kDa.
Western Blot of Rabbit anti-NOTCH2 (Cleaved N terminal) antibody. Lane 1 Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 2: U-251 Lysate (p/n W09-001-GY4). Lane 3: PC-3 Lysate (p/n W09-001-GV6). Lane 4: MCF-7 Lysate (p/n W09-000-360). Lane 5: Hep-G2 Lysate (p/n W09-001-GJ5). Lane 6: A431 Lysate (p/n W09-000-361). Lane 7: A549 Lysate (p/n W09-001-372). Lane 8: J774A.1 Lysate (p/n W10-001-GX3). Lane 9: C2C12 Lysate (p/n W10-001-GL7). Lane 10: Mouse Embryonic Fibroblast Lysate (p/n W10-001-371). Lane 11: NIH/3T3 Lysate (p/n W10-000-358). Lane 12: PC-12 Lysate (p/n W12-001-GL9). Lane 13: Rat Brain Lysate (p/n W12-000-T077). Lane 14: Mouse Brain Lysate (p/n W10-000-T004). Lane 15 Marker: Opal Pre-stained Ladder. Load: 10 ug of lysate/lane. Primary antibody: NOTCH2 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted size: ~86kDa in human cell lines (2-7) for NOTCH2.
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Rockland's anti NOTCH 2 antibody was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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メーカー |
品番 |
包装 |
RKL
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100-401-408
|
200 UL
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※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
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保存温度 |
-20℃
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