別品名 |
AGS 2 antibody, AGS2 antibody, hN2 antibody, N2 antibody, Neurogenic locus notch homolog protein 2 antibody, Notch 2 antibody, Notch 2 intracellular domain antibody, Notch homolog 2 antibody
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抗原部位 |
a.a.2396-2409, N-terminus
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Serum
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry
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免疫動物 |
Rabbit
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交差種 |
Human
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GENE ID |
4853
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Accession No.(Gene/Protein) |
Q04721
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Gene Symbol |
NOTCH2
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]18418349, 19433762, 19828677
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Rockland's Anti Notch 2 antibody was diluted 1:500 to detect NOTCH 2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Western blot using Rockland's anti-Notch 2 (intra) antibody shows detection of a band at ~110 kDa corresponding to active Notch 2 protein. Western Blot analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.
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Rockland's Anti Notch 2 antibody was diluted 1:500 to detect NOTCH 2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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メーカー |
品番 |
包装 |
RKL
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100-401-406
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200 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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