別品名 |
NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Anti-NF-kB antibody
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抗原部位 |
QLRRPpSDRELSC
|
種由来 |
Human
|
標識物 |
Unlabeled
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精製度 |
Serum
|
適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry
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免疫動物 |
Rabbit
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交差種 |
Human
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翻訳後修飾 |
リン酸化
|
GENE ID |
5970
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Accession No.(Gene/Protein) |
Q04206
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Gene Symbol |
RELA
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]27471270, 15531529, 16300481, 20204068, 18320069, 22445500, 26197393
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Rockland's anti p65 pS276 antibody was diluted 1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Rockland Immunochemical's pan reactive anti p65 (code# 100-4165) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method. Other detection methods will yield similar results. Personal Communication, Aggarwal BB
Anti-pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method. Other detection methods will yield similar results. Data contributed by Yu, M., NIH, personal communication.
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Rockland's anti p65 pS276 antibody was diluted 1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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メーカー |
品番 |
包装 |
RKL
|
100-401-264
|
100 UL
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
保存温度 |
-20℃
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