Anti IKK β  

Immunohistochemistry of Anti-IKKs antibody. Tissue: human placenta was formalin fixed and paraffin embedded. No pre-treatment of sample was required. Primary Antibody: Anti-IKKs was diluted 1:500 to detect IKKs in tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Western blot of anti-IKKs antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.