| 別品名 |
Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Serum
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| 適用 |
Western Blot
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
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| GENE ID |
1027
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| Accession No.(Gene/Protein) |
P46527
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| Gene Symbol |
CDKN1B
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Keller,U.B., Old,J.B., Dorsey,F.C., Nilsson,J.A., Nilsson,L., MacLean,K.H., Chung,L., Yang,C., Spruck,C., Boyd,K., Reed,S.I. and Cleveland,J.L. (2007) Myc targets Cks1 to provoke the suppression of p27Kip1, proliferation and lymphomagenesis. EMBO J. 26 (10), 2562-2574. Naidu,R., Har,Y.C. and Taib,N.A. (2007) P27 V109G Polymorphism is associated with lymph node metastases but not with increased risk of breast cancer. J. Exp. Clin. Cancer Res. 26 (1), 133-140. Pertia,A., Nikoleishvili,D., Trsintsadze,O., Gogokhia,N., Managadze,L. and Chkhotua,A. Prognostic significance of p27(Kip 1) expression in renal cell Carcinoma. Georgian Med News 143, 12-17.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Rabbit anti p27 antibody. Lane 1: HEK293 pcDNA3. Lane 2: HEK293 pcDNA3 Rb2wt. Lane 3: HEK293 pcDNA3 Rb2S941A. Lane 4: HEK293 pcDNA3 Rb2 PM19. Load: 30 ug per lane. Primary antibody: p27 antibody diluted in 1%BSA at 1:2000 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO (p/n B501 0500) overnight at 4C. Predicted/Observed size: 27 kDa for p27.
Western blot using Rockland's affinity purified anti-p27 antibody shows detection (lanes 1-3) of p27 protein in MCF7 whole cell lysate (p/n W09-000-360) (arrowhead).？ Separation was achieved using a 4-20% gradient gel.？ Blocking occurred using 5% BLOTTO (p/n B501-0500).？ Primary antibody was diluted 1:500 in 1% BLOTTO.？ The membrane was washed and reacted with a 1:10,000 dilution of DylightTM 800 conjugated Gt-a-Rabbit IgG (p/n 611-145-002).？ Molecular weight estimation was made by comparison to prestained MW markers p/n MB-210-0500) indicated at the left (lane M).？ Other detection systems will yield similar results.
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Western Blot of Rabbit anti p27 antibody. Lane 1: HEK293 pcDNA3. Lane 2: HEK293 pcDNA3 Rb2wt. Lane 3: HEK293 pcDNA3 Rb2S941A. Lane 4: HEK293 pcDNA3 Rb2 PM19. Load: 30 ug per lane. Primary antibody: p27 antibody diluted in 1%BSA at 1:2000 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO (p/n B501 0500) overnight at 4C. Predicted/Observed size: 27 kDa for p27.
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| メーカー |
品番 |
包装 |
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RKL
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100-401-172
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100 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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