Anti EGFR  

Immunohistochemistry of Anti-EGFR Antibody with positive staining.

Western blot using Rockland's anti-EGFR antibody. Lane 1: unstimulated A431 whole cell lysates (p/n W09-000-361). Lane 2: EGF stimulated A431 whole cell lysates (p/n W09-000-362). Shows detection of a band at ~170 kDa corresponding to human EGFR present in unstimulated and stimulated (50 ng/ml for 15 min) lysates (arrowhead). Loaded: 30μg lysate was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. Primary Antibody: Anti-EGFR at 1:1,000 overnight at 4° C. Secondary Antibody: IRDye^(R) 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (p/n 611-132-122) at 1:10,000 dilution of for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye^(R) 800 fluorescence image was captured using the Odyssey^(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Combined immunoprecipitation and western blot using anti-EGFR antibody. Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15' at 100 ng/ml and without (-) the addition of EGF. The combination of immunoprecipitation and western blotting was performed using the anti-EGFR antibody for immunoprecipitation (10 μL) followed by western blot detection using an anti-phosphotyrosine antibody (Panel A). This was repeated in reverse order using a 1:2000 dilution of anti-EGFR for western blot (Panel B). Visualization occurred using an ECL system. Film exposure was approximately 1’. Other detection systems will yield similar results.

Expression of corneal epithelial gene markers. (B) The relative protein expression levels of EGFR (p/n 100-401-149), involucrin and TG1 as determined by Western blot assay; β-actin (p/n 600-401-886) was used as a loading control, and blocking buffer (p/n MB-070). Fig 3. PMID:？19638217