別品名 |
Receptor tyrosine-protein kinase erbB-1 antibody, c-erbB-1 antibody
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抗原部位 |
C-terminus
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Serum
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適用 |
Western Blot Immunohistochemistry Immunoprecipitation
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免疫動物 |
Rabbit
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交差種 |
Human Rat
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GENE ID |
1956
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Accession No.(Gene/Protein) |
P00533
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Gene Symbol |
EGFR
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]17130473, 24463098, 27065097, 19638217, 27260220
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry of Anti EGFR Antibody with positive staining.
Combined immunoprecipitation and western blot using anti-EGFR antibody. Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15' at 100 ng/ml and without (-) the addition of EGF. The combination of immunoprecipitation and western blotting was performed using the anti-EGFR antibody for immunoprecipitation (10 μL) followed by western blot detection using an anti-phosphotyrosine antibody (Panel A). This was repeated in reverse order using a 1:2000 dilution of anti-EGFR for western blot (Panel B). Visualization occurred using an ECL system. Film exposure was approximately 1’. Other detection systems will yield similar results.
Western blot using Rockland's anti-EGFR antibody. Lane 1: unstimulated A431 whole cell lysates (p/n W09-000-361). Lane 2: EGF stimulated A431 whole cell lysates (p/n W09-00-362). Shows detection of a band at ~170 kDa corresponding to human EGFR present in unstimulated and stimulated (50 ng/ml for 15 min) lysates (arrowhead). Loaded: 30ug lysate was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. Primary Antibody: Anti-EGFR at 1:1,000 overnight at 4 C. Secondary Antibody: IRDyeR 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (p/n 611-132-122) at 1:10,000 dilution of for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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Immunohistochemistry of Anti EGFR Antibody with positive staining.
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メーカー |
品番 |
包装 |
RKL
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100-401-149
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250 UL
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※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
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保存温度 |
-20℃
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