別品名 |
A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibod
|
種由来 |
Human
|
由来詳細 |
Plasma
|
標識物 |
Unlabeled
|
精製度 |
Serum
|
適用 |
Western Blot
|
免疫動物 |
Goat
|
交差種 |
Human
|
GENE ID |
5265
|
Accession No.(Gene/Protein) |
NP_000286, P01009
|
Gene Symbol |
SERPINA1
|
形状 |
凍結乾燥品
|
[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Rockland Goat anti Alpha 1 Anti Trypsin (100 101 147 lot 5842, red), Rabbit anti Transferrin (109 4134 lot 3033), and Mouse a GST (200 301 200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS PAGE, transferred to 0.2 um PVDF using the BioRad Trans Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB 070 over night at 4C). Detection shown was using DyLight549 Donkey anti Rabbit IgG (611 742 127 lot 21100, shown as green) ? DyLight 488 Donkey anti Mouse IgG (610 741 124 lot 21095, shown as blue), ?and DyLight 649 Donkey anti Goat IgG (605 743 125 lot 20834, shown as red) at 1:10000 (in MB 070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
Rockland primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (1° 109-4134, green 2° 611-743-127); Alpha 1 anti trypsin (1° 100-101-147, red 2° 605-742-125); and Human IgG (1° 109-3102, Blue 2° 610-741-124 in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in Blocking Buffer for Fluorescent Western Blotting - MB-070 and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in MB-070 and incubated ~ 1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Biorad VersaDoc4000.
Rockland Goat anti Alpha-1anti-Trypsin antibody (200-101-147 lot 5854) was used to detect Alpha-1anti-Trypsin under reducing (R) conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.
|
|
Rockland Goat anti Alpha 1 Anti Trypsin (100 101 147 lot 5842, red), Rabbit anti Transferrin (109 4134 lot 3033), and Mouse a GST (200 301 200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS PAGE, transferred to 0.2 um PVDF using the BioRad Trans Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB 070 over night at 4C). Detection shown was using DyLight549 Donkey anti Rabbit IgG (611 742 127 lot 21100, shown as green) ? DyLight 488 Donkey anti Mouse IgG (610 741 124 lot 21095, shown as blue), ?and DyLight 649 Donkey anti Goat IgG (605 743 125 lot 20834, shown as red) at 1:10000 (in MB 070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
|
|
|
メーカー |
品番 |
包装 |
RKL
|
100-101-147
|
2 ML
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
保存温度 |
4℃
|
|