Collagen Type IV  

SDS-PAGE Results of Human Collagen Type IV. Lane 1: Human Collagen Type IV Reduced (5.0μg). Lane 2: Opal Prestained Molecular Weight Protein (p/n MB-210-0500). Lane 3: Human Collagen Type IV Non-Reduced (5.0μg). 4-20% Gel, Coomassie Stained. Observed MW: Type IV α1, α2, α3 bands.

Knockdown of NORAD inhibits proliferation, inflammation and fibrosis in HG-induced HMCs. Levels of (G) Col. IV in HMCs measured via ELISA.？**P<0.01 vs. NG;？^{P<0.01 vs. HG + si-NC. NORAD, non-coding RNA activated by DNA damage; NG, normal glucose; HG, high glucose; HMCs, human mesangial cells; si, small interfering RNA; NC, negative control Col. IV, type IV collagen. Figure 2. PMID: 34194552.}

NORAD reverses the inhibitory effects of miR-485 on HG-induced HMCs. (F) Contents of Col. IV, FN and PAI-1 in HG-induced HMCs measured via ELISA？**P<0.01 vs. miR-NC;？#P<0.05 vs. miR-485 mimics. miR, microRNA; NORAD, non-coding RNA activated by DNA damage; HG, high glucose; HMCs, human mesangial cells; ov, overexpression vector; NC, negative control; Col. IV, type IV collagen; FN, fibronectin; PAI-1, plasminogen activator inhibitor 1. Figure 4. PMID: 34194552.

NORAD knockdown inhibits proliferation, inflammation and fibrosis in HG-induced HMCs by regulating the miR-485/NRF1 axis. (G) Contents of Col. IV, FN and PAI-1 in HG-induced HMCs as determined via ELISA.？**P<0.01 vs. si-NC;？#P<0.05 vs. si-NORAD + inhibitor NC;？&P<0.05 vs. si-NORAD + ov-NC. miR, microRNA; NORAD, non-coding RNA activated by DNA damage; NRF1, nuclear respiratory factor 1; HG, high glucose; HMCs, human mesangial cells; ov, overexpression vector; si, small interfering RNA; NC, negative control; Col. IV, type IV collagen; FN, fibronectin; PAI-1, plasminogen activator inhibitor 1. Figure 6. PMID: 34194552.