Collagen Type I  

SDS-PAGE Results of Bovine Collagen Type I.Lane 1: Bovine Collagen Type I -Reduced [10μg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500)Lane 3: Bovine Collagen Type I -Non-Reduced [10μg]. 4-20% Gel, Coomassie Stained.

Aggf1 interacts with and modulates SMAD7 activity.(D) Primary HSCs were infected with Ad-V or Ad-AGGF1 and then transfected with either siRNA targeting SMAD7 or scrambled siRNA (SCR). Expression of fibrogenic genes was examined by Western and qPCR (not shown). Figure 4. PMID: 26850475.

Aggf1 suppresses liver fibrosis following injury in mice. (B) C57/BL6 mice were injected via tail vein adenovirus carrying either Aggf1 expression vector (Ad-AGGF1) or an empty vector (Ad-V) followed by CCl4 injection to induce liver injury. Expression of fibrogenic genes was examined by Western and qPCR (not shown). Figure 2. PMID: 26850475.

C57/BL mice were injected peritoneally with chaetocin (25mg/kg) or DMSO 2 days prior to the LAD procedure. The mice were sacrificed 4 weeks after the surgery. (B) Expression of pro-fibrogenic genes was examined by Western blotting and qPCR (not Shown). Supplementary Fig.9. PMID: 28361889.