Anti XBP-1

Figure 1 Western Blot Validation in Human HepG2 Cell Lysate
Loading: 15 μg of lysates per lane.Antibodies: XBP-1 3687 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) the absence and (B) the presence of blocking peptide

Figure 2 Western Blot Validation with Recombinant Protein
Loading: 100 ng of human XBP-1 recombinant protein per lane.Antibodies: XBP-1 3687 (A: 0.5 μg/mL, B: 1 μg/mL and C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 23kD.

Figure 3 Western Blot Validation of XBP-1 in A549 Cells
Loading: 15 μg of A549 cell lysateAntibodies: XBP-1 3687, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane1: 0.25 μg/mLLane2: 0.5 μg/mLLane3: 1 μg/mL

Figure 4 Western Blot Validation of XBP-1 in HepG2 Cells
Loading: 15 μg of HepG2 cell lysateAntibodies: XBP-1 3687, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane1: 1 μg/mLLane2: 2 μg/mL

Figure 5 Immunofluorescence Validation of XBP-1 in Human HepG2 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling XBP-1 with 3687 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 6 Immunofluorescence Validation of XBP-1 in Human Pancreas Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling XBP-1 with 3687 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 7 Immunofluorescence Validation of XBP-1 in Human Liver Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Liver Tissue labeling XBP-1 with 3687 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 8 Immunofluorescence Validation of XBP-1 in Mouse Pancreas Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling XBP-1 with 3687 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 9 Immunohistochemistry Validation of XBP-1 in Mouse Spleen Tissue
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-XBP-1 antibody (3687) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 10 Immunohistochemistry Validation of XBP-1 in Mouse Testis Tissue
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-XBP-1 antibody (3687) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.