別品名 |
MD-2 Antibody: MD2, MD-2, ly-96, ESOP-1, ESOP1, MD2, Lymphocyte antigen 96, Ly-96
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot IHC paraffin embedding section Enzyme Linked Immunosorbent Assay Immuno Fluorescence
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse Rat
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GENE ID |
23643
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Accession No.(Gene/Protein) |
NP_056179
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Gene Symbol |
LY96
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形状 |
液状
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推奨品 |
ポジティブコントロール 品番:1406 - Mouse Spleen Tissue Lysate ポジティブコントロール 品番:1466 - Rat Spleen Tissue Lysate
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その他 |
[Protein GI Number]223555998 [Swiss-Prot No]Q9Y6Y9
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参考文献 |
O'Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
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Figure 1 Western Blot Validation with Recombinant Protein Loading: 30 ng of human MD-2 recombinant protein per lane.Antibodies: MD-2, 3289 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 2 Western Blot Validation in Daudi Cell Lysate Loading: 15 μg of lysates per lane.Antibodies: MD-2, 3289 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 3 Western Blot Validation in Mouse Spleen Tissue Lysate Loading: 15 μg of lysates per lane.Antibodies: MD-2, 3289 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 4 Western Blot Validation in Mouse Tissue Lysates Loading: 15 μg of lysates per lane.Antibodies: MD-2, 3289 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 5 Western Blot Validation in Rat Spleen Tissue Lysate Loading: 15 μg of lysates per lane.Antibodies: MD-2, 3289 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 6 Western Blot Validation in Rat Tissue Lysates Loading: 15 μg of lysates per lane.Antibodies: MD-2, 3289 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 7 Immunohistochemistry Validation of MD-2 in Human Spleen Tissue Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MD-2 antibody (3289) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 8 Immunofluorescence Validation of MD-2 in Human Spleen Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling MD-2 with 3289 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Figure 9 Immunohistochemistry Validation of MD-2 in Rat Spleen Tissue Immunohistochemical analysis of paraffin-embedded rat spleen using anti-MD-2 antibody (3289) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 10 Immunofluorescence Validation of MD-2 in Rat Spleen Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed rat spleen tissue labeling MD-2 with 3289 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Figure 1 Western Blot Validation with Recombinant Protein Loading: 30 ng of human MD-2 recombinant protein per lane.Antibodies: MD-2, 3289 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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メーカー |
品番 |
包装 |
PSC
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3289
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100 UG [1 mg/mL]
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※表示価格について
当社在庫 |
なし
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納期目安 |
3週間程度
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保存温度 |
-20℃
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