別品名 |
IRAK-4 Antibody: IPD1, REN64, IRAK-4, NY-REN-64, Interleukin-1 receptor-associated kinase 4, Renal carcinoma antigen NY-REN-64
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抗原部位 |
C-terminus
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immuno Fluorescence Immunocytochemistry (cell)
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human
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GENE ID |
51135
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Accession No.(Gene/Protein) |
AAM15772
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Gene Symbol |
IRAK4
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形状 |
液状
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推奨品 |
ポジティブコントロール 品番:1204 - K562 Cell Lysate
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その他 |
[Protein GI Number]20219010 [Swiss-Prot No]Q9NWZ3
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参考文献 |
Cao Z, Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31. Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5 Wesche H, Gao X, Li X, et al. IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family. J. Biol. Chem. 1999; 274:19403-10. Li S, Strelow A, Fontana EJ, et al. IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase. Proc. Natl. Acad. Sci. USA 2002; 99:5567-72.
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Figure 1 Western Blot Validation in Human Cell Lines Loading: 15 μg/ of lysates per lane.Antibodies: IRAK4 3125 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 μg of lysates per lane.Antibodies: IRAK4-3125 (1 μg/mL), IRAK4-24-025 (1 μg/mL), beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 3 Western Blot Validation in Human HeLa Cell Lines Loading: 15 μg of lysates per lane.Antibodies: IRAK4 3125, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 μg/mLLane 2: 2 μg/mLLane 3: 4 μg/mL
Figure 4 Immunocytochemistry Validation of IRAK4 in K562 Cells Immunocytochemical analysis of K562 cells using anti-IRAK4 antibody (3125) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 5 Immunofluorescence Validation of IRAK4 in K562 Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 Cells labeling IRAK4 with 3125 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Figure 6 KO and KD Validation of IRAK4 in Mouse Bone Marrow-derived Macrophages (BMDM) (Koziczak-Holbro et al., 2008) Western blot analysis with anti-IRAK4 antibodies was performed for IRAK4 in BMDM isolated from the mice. IRAK4 expression was not observed in the IRAK-4-/- cells and also reduced in IRAK4 mutant (IRAK-4 KD) when compared with WT.
Figure 7 Regulated Expression Validation of IRAK4 in Mouse RAW 264 Cells (Hatao et al., 2004) RAW 264 cells were stimulated with (a) CSK4 and (b) CpG-DNA in the absence or presence of proteasome inhibitors (MG132 and Lactactstin). When detected with anti-IRAK4 antibodies (C12 from ProSci, Inc.), IRAK4 expression was found to be reduced without the inhibitors. The smaller protein band, a cleavage product of IRAK4, was present in the absence of both inhibitors.
Figure 8 KD Validation of IRAK4 in HEK293T Cells (Heinz et al., 2012) Western blot analysis with anti-IRAK4 antibodies was performed for IRAK4 in HEK293T cells. IRAK4 expression was not observed in IRAK4 knockdown cells.
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Figure 1 Western Blot Validation in Human Cell Lines Loading: 15 μg/ of lysates per lane.Antibodies: IRAK4 3125 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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メーカー |
品番 |
包装 |
PSC
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3125
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100 UG [1 mg/mL]
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※表示価格について
当社在庫 |
なし
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納期目安 |
3週間程度
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保存温度 |
-20℃
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