別品名 |
AIF Antibody: CNK, KSR, CNK1, Connector enhancer of kinase suppressor of ras 1, CNK homolog protein 1, Connector enhancer of KSR 1
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot IHC paraffin embedding section Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse Rat
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GENE ID |
9131
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Accession No.(Gene/Protein) |
O95381
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Gene Symbol |
AIFM1
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形状 |
液状
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推奨品 |
ポジティブコントロール 品番:1204 - K562 Cell Lysate ポジティブコントロール 品番:1461 - Rat Heart Tissue Lysate ポジティブコントロール 品番:1401 - Mouse Heart Tissue Lysate
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その他 |
[Protein GI Number]50400606 [Swiss-Prot No]O95381
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参考文献 |
Zamzami N and Kroemer G. Condensed matter in cell death. Nature 1999; 401:127-8. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999; 397:441-6. Daugas E, Susin SA, Zamzami N, et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 2000; 14:729-39.
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Figure 1 Western Blot Validation in Different Species Loading: 15 μg of lysates per lane.Antibodies: AIF 2267, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Human K562 cellsLane B: Rat heartLane C: Mouse heart
Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 μg of lysates per lane.Antibodies: AIF 2267, (1 μg/mL), AIF 2239, (1 μg/mL), AIF 2301, (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 3 Western Blot Validation in Human Cell Lines Loading: 15 μg of lysates per lane.Antibodies: AIF 2267, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 4 Western Blot Validation in Mouse and Rat Cell Lines Loading: 15 μg of lysates per lane.Antibodies: AIF 2267, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 5 Immunohistochemistry Validation of AIF in Human Retina Tissue Immunohistochemical analysis of paraffin-embedded human retina tissue using anti-AIF antibody (2267) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 6 KD and Induced Validation of AIF in H1299 Cells(Stambolsky et al., 2006) Western blot analysis of AIF knockdown with anti-AIF antibodies in H1299 cells. AIF expression was disrupted in AIF knockdown cells (siRNA1 and siRNA4). An increased expression of AIF was induced by ZnCl2 treatment, which was not observed in AIF knockdown cells.
Figure 7 KD Validation of AIF in AIF Silenced Stable Cells(Apostolova et al., 2006) AIF silencing is sustained in stable cell lines. Western blot analysis ofstable lines AIF-1-10, AIF-2-4 and pU6-2 using anti-AIF antibodies. AIF protein was disrupted after AIF silencing with AIF siRNA (AIF-1-10 and AIF-2-4) as compared to control (Hep3B and pU6-2).
Figure 8 Immunofluorescence Validation of AIF in HeLa Cells (Rossi et al., 2009) HeLa cells were transfected withMitofilin-specific (MITO-siRNA) or with nonspecific (NS-siRNA) siRNAs. AIF staining with anti-AIF antibodies was shown as a mitochondrial marker in the absence of Mitofilin.
Figure 9 Immunofluorescence Validation of AIF in Rat Hippocampal Neurons (Hofer et al., 2011) (G-L) After exposure to bacterial components, AIF colocalized in mature neurons (MAP2; I, L), immature neurons (DcX;H, K), and stem/progenitor cells (Nestin; G, J). AIF expression was detected by anti-AIF antibodies.
Figure 10 Subcellular Localization Validation of AIF in mononuclear cells (Gupta et al., 2003) A shows mononuclear cells (MNCs) alone, B shows MNCs transfected with control plasmid, C shows MNCs transfected with Bcl-2 expression plasmid. Overlay is of Mitotracker (red) and AIF (green). Hoechst 33258 dye is used to examine chromatin fragmentation. The release of AIF form mitochondria is detected by anti-AIF antibodies.
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Figure 1 Western Blot Validation in Different Species Loading: 15 μg of lysates per lane.Antibodies: AIF 2267, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Human K562 cellsLane B: Rat heartLane C: Mouse heart
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メーカー |
品番 |
包装 |
PSC
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2267
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100 UG [1 mg/mL]
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※表示価格について
当社在庫 |
なし
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納期目安 |
3週間程度
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保存温度 |
-20℃
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