| 別品名 |
LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223
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| 抗原部位 |
extracellular domain
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| 種由来 |
Human
|
| 標識物 |
Unlabeled
|
| 精製度 |
Ig fraction - Protein A
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| 適用 |
Western Blot
IHC paraffin embedding section
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Immunocytochemistry (cell)
Flow Cytometry
|
| 免疫動物 |
Mouse
|
| 抗体クラス |
IgG1κ
|
| クローン |
2G8
|
| 交差種 |
Human
|
| GENE ID |
3902
|
| Accession No.(Gene/Protein) |
NP_002277
|
| Gene Symbol |
LAG3
|
| 形状 |
液状
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| その他 |
[Protein GI Number]167614500
[Swiss-Prot No]P18627
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| 参考文献 |
Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216-21.
Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619-22.
Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885-91.
Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970-9.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot analysis of LAG-3 in over expressing HEK293 cells using LAG-3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.
Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml.
Green: LAG3 Antibody [2G8] (RF16082)
Blue: DAPI staining
Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml.
Red: LAG3 Antibody [2G8] (RF16082)
Blue: DAPI staining
Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.
Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.
Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with RF16082, RF16083, RF16084, RF16086, RF16087, RF16088, and RF16089 antibodies at decreasing concentrations.
A sandwich ELISA was performed using the anti-LAG3 mAbs RF16082, RF16083, RF16084, RF16086, RF16087 and RF16088 as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.
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Western blot analysis of LAG-3 in over expressing HEK293 cells using LAG-3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.
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|
| メーカー |
品番 |
包装 |
|
PSC
|
RF16082
|
100 UG
[1 mg/mL]
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※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
3週間程度
|
| 保存温度 |
-２０℃
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