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Anti-GFP Antibody - Western Blot. Western blot of GFP recombinant protein detected with monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. Mab anti-GFP detects a 27 kD band corresponding to the epitope tag GFP. The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1.0 mg/ml for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] MX10 (. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-GFP Chemiluminescent Kit for Western blot. Known amounts of recombinant GFP protein ( were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4? prior to probing the blot with the anti-GFP monoclonal antibody Anti-GFP (MOUSE) Monoclonal Antibody - 600-301-215 diluted 1:1000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n LS-C60772) was performed at a dilution of 1:20000 for 1h at 4?. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).
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Anti-GFP Antibody - Western Blot. Western blot of GFP recombinant protein detected with monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. Mab anti-GFP detects a 27 kD band corresponding to the epitope tag GFP. The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1.0 mg/ml for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] MX10 (. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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| 別品名 |
GFP
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| 種由来 |
Aequorea victoria
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| 交差種 |
Aequorea victoria
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
Flow Cytometry
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| 免疫動物 |
Mouse
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| クローン |
9F9.F9
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| 抗体クラス |
IgG1κ
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| Gene Symbol |
GFP
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| メーカー |
品番 |
包装 |
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LSP
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LS-C154208-1
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1 MG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約1ヶ月
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| 保存温度 |
-２０℃
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