Fluorescein di-β-D-galactopyranoside (FDG)  

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CAS RN® 17817-20-8
使用目的 Ultra-sensitive substrate for lacZ β-galactosidase detection in vivo and in vitro; useful in one of the most common transfection optimization assays in molecular biology.
参考文献 Johnson I. (1998) "Fluorescent probes for living cells." Histochem J 30: 123.
Rakhmanova VA, MacDonald RC. (1998) "A microplate fluorimetric assay for transfection of the beta-galactosidase reporter gene." Analytical Biochem 257: 234.
Poot M, Arttamangkul S. (1997) "Verapamil inhibition of enzymatic product efflux leads to improved detection of beta-galactosidase activity in lacZ-transfected cells." Cytometry 28: 36.
Nirenberg S, Meister M. (1997) "The light response of retinal ganglion cells is truncated by a displaced amacrine circuit." Neuron 18(4): 637-50.
Craig D, et al. (1995) "Fluorescence-based enzymatic assay by capillary electrophoresis laser-induced fluorescence detection for the determination of a few beta-galactosidase molecules." Analalytical Biochem 226: 147.
M, Molina M, Nombela C. (1994) "Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells." Appl Environ Microbiol 60(12): 4638-4641.
Lin S, Yang S, Hopkins N. (1994) “lacZ expression in germline transgenic zebrafish can be detected in living embryos." Dev Biol 161: 77.
Fiedler F, Hinz H. (1994) "No intermediate channelling in stepwise hydrolysis of fluorescein di-beta-D-galactoside by beta-galactosidase." Eur J Biochem 222: 75.
Klunder I, Hulser DF. (1993) "Beta-galactosidase activity in transfected Ltk-cells is differentially regulated in monolayer and in spheroid cultures." Exp Cell Res 207: 155.
Russo-Marie F, et al. (1993) "Beta-galactosidase activity in single differentiating bacterial cells." Proc Natl Acad Sci USA 90: 8194.
Saalmuller A, Mettenleiter TC. (1993) "Rapid identification and quantitation of cells infected by recombinant herpesvirus (pseudorabies virus) using a fluorescence-based beta-galactosidase assay and flow cytometry." J Virol Methods 44: 99.
Nirenberg S, Cepko C. (1993) "Targeted ablation of diverse cell classes in the nervous systemin vivo." J Neurosci 13(8): 3238-51.
Alvarez AM, Ibanez M, Rotger R. (1993) "Beta-galactosidase activity in bacteria measured by flow cytometry." BioTechniques 15: 974.
Roederer M, Fiering S, Herzenberg LA. (1991) "FACS-Gal: flow cytometric analysis and sorting of cells expressing reporter gene constructs." Methods: Comp Meth Enzymol 2: 248.
Kerr WG, Herzenberg LA. (1991) "Gene-search viruses and FACS-Gal permit the detection, isolation, and characterization of mammalian cells with in situ fusions between cellular genes and escherichia coli lacZ." Methods: Comp Meth Enzymol 2: 261.Emilie D, et al. (1989) "Visualizing interleukin 2 gene expression at the single cell level.” Eur J Immunol 19: 1619.
Jongkind JF, Verkerk A, Sernetz M. (1986) "Detection of acid-beta-galactosidase activity in viable human fibroblasts by flow cytometry." Cytometry 7: 463.Yashphe J, Halvorson HO. (1976) "Beta-D-galactosidase activity in single yeast cells during cell cycle of Saccharomyces lactis." Science 191: 1283.
Kato K, et al. (1975) "Enzyme-linked immunoassay I. Novel method for synthesis of the insulin-beta-D-galactosidase conjugate and its applicability for insulin assay." J Biochem 78: 235.
Rotman B, Zderic JA, Edelstein M. (1963) "Fluorogenic Substrates for beta-D-galactosidases and phosphatases derived from fluorescein (3,6-dihydroxyfluoran) and its monomethyl ether." Proc Natl Acad Sci USA 50: 1.

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Fluorescein di-β-D-galactopyranoside (CAS 17817-20-8):Fluorescein di-galactoside; FDG

メーカー 品番 包装
MGT M0250 5 MG
希望販売価格 ¥43,000

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納期目安 約10日
保存温度 -20℃

 

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メーカー名 Marker Gene Technologies, Inc.
略号 MGT
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