Puromycin Dihydrochloride  

別品名 Stylomycin Hydrochloride, (3'-[[(2S)-2-Amino-3-(4-methoxyphenyl)-1- oxopropyl]amino]-3'-deoxy-N,N-dimethyl-adenosine
別包装 あり
純度 >=98%
分子量 544.43
CAS RN® 58-58-2
化合物の概要 Protein synthesis inhibitor, DPP2 and metallopeptidase inhibitor.
構造 C22H29N7O5 2HCl
溶媒 Soluble in water.
使用目的 Puromycin is an aminonucleoside antibiotic compound originally produced by Streptomyces alboniger. Puromycin displays antibacterial activity through inhibition of ribosomal protein translation; it resembles the 3’ end of tRNA and is incorporated into growing protein chains through the ribosomal A site, inducing premature chain termination. Puromycin also induces DNA damage mediated by ROS and oxidative stress in animal models. In vitro, puromycin inhibits insulin-stimulated glycolysis by inhibiting insulin activation of phosphofructokinase 2. Puromycin also inhibits dipeptidyl peptidase II (DPP2; serine peptidase) and metallopeptidase. Additionally, this compound induces ERK activation-dependent apoptosis and mTOR-dependent autophagy in podocytes, leading to proteinuria and glomerular damage.
その他 [融点]175.5℃-177℃
[外観]White Powder
参考文献 Kang YL, Saleem MA, Chan KW, et al. The cytoprotective role of autophagy in puromycin aminonucleoside treated human podocytes. Biochem Biophys Res Commun. 2014 Jan 10;443(2):628-34. PMID: 24333414.Liu S, Ding J, Fan Q, et al. The activation of extracellular signal-regulated kinase is responsible for podocyte injury. Mol Biol Rep. 2010 Jun;37(5):2477-84. PMID: 19728154.Marshall CB, Pippin JW, Krofft RD, et al. Puromycin aminonucleoside induces oxidant-dependent DNA damage in podocytes in vitro and in vivo. Kidney Int. 2006 Dec;70(11):1962-73. PMID: 17035936.Probst I, Quentmeier A, Schweickhardt C, et al. Stimulation by insulin of glycolysis in cultured hepatocytes is attenuated by extracellular ATP and puromycin through purine-dependent inhibition of phosphofructokinase 2 activation. Eur J Biochem. 1989 Jun 15;182(2):387-93. PMID: 2525468.


メーカー 品番 包装
LKT P8168 25 MG
希望販売価格 ¥16,000


当社在庫 なし
納期目安 1週間程度
保存温度 4℃




メーカー名 LKT Laboratories, Inc.
略号 LKT