別品名 |
Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry Flow Cytometry Immunoprecipitation
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免疫動物 |
Rabbit Mono
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抗体クラス |
IgG
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クローン |
8D1
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交差種 |
Human
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Accession No.(Gene/Protein) |
Q16611
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形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, Colo320 whole cell lysate All lanes: BAK1 antibody at 0.9ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24, 17 KDa Observed band size: 24 KDa
IHC image of CSB-RA624111A0HU diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA624111A0HU diluted at 1:90 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating BAK1 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA624111A0HU in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA624111A0HU (3ug) + HEK293 whole cell lysate (500ug) Lane 3: HEK293 whole cell lysate (20ug)
Overlay histogram showing Hela cells stained with CSB-RA624111A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, Colo320 whole cell lysate All lanes: BAK1 antibody at 0.9ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24, 17 KDa Observed band size: 24 KDa
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メーカー |
品番 |
包装 |
CSB
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CSB-RA624111A0HU
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100 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
2週間程度
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保存温度 |
-20℃
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