別品名 |
Cyclic AMP-dependent transcription factor ATF-4, cAMP-dependent transcription factor ATF-4, Activating transcription factor 4, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, DNA-binding protein TAXREB67, Tax-responsive enhancer element-binding protein 67, TaxREB67, ATF4, CREB2, TXREB
|
種由来 |
Human
|
標識物 |
Unlabeled
|
精製度 |
Affinity Purified
|
適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry Immuno Fluorescence Flow Cytometry Immunoprecipitation
|
免疫動物 |
Rabbit Mono
|
抗体クラス |
IgG
|
クローン |
9E1
|
交差種 |
Human
|
Accession No.(Gene/Protein) |
P18848
|
形状 |
液状
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: ATF4 antibody at 1.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 KDa Observed band size: 50 KDa
IHC image of CSB-RA002272A0HU diluted at 1:160 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-RA002272A0HU at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating ATF4 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA002272A0HU in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA002272A0HU (3ug) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug)
Overlay histogram showing Hela cells stained with CSB-RA002272A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
|
|
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: ATF4 antibody at 1.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 KDa Observed band size: 50 KDa
|
|
|
メーカー |
品番 |
包装 |
CSB
|
CSB-RA002272A0HU
|
50 UL
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
2週間程度
|
保存温度 |
-20℃
|
|