別品名 |
2610001F05Rik antibody; AW045757 antibody; MGC118982 antibody; MGC118983 antibody; RGD1565585 antibody; RP23-356J7.1 antibody; SDP8 antibody; SNX 12 antibody; SNX12 antibody; SNX12_HUMAN antibody; Sorting nexin 12 antibody; Sorting nexin-12 antibody
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抗原部位 |
a.a.2-172
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Ig fraction - Protein G
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry Immuno Fluorescence Immunoprecipitation
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human
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Accession No.(Gene/Protein) |
Q9UMY4
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形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Positive WB detected in: PC-3 whole cell lysate, A549 whole cell lysate All lanes: SNX12 antibody at 2.83μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20, 19 kDa Observed band size: 20 kDa
IHC image of CSB-PA891787LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CSB-PA891787LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating SNX12 in A549 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA891787LA01HU in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA891787LA01HU (6μg) + A549 whole cell lysate (500μg) Lane 3: A549 whole cell lysate (20μg)
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Western Blot Positive WB detected in: PC-3 whole cell lysate, A549 whole cell lysate All lanes: SNX12 antibody at 2.83μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20, 19 kDa Observed band size: 20 kDa
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メーカー |
品番 |
包装 |
CSB
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CSB-PA891787LA01HU
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
2週間程度
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保存温度 |
-20℃
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