別品名 |
Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, CD21, CR2, C3DR
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry Immuno Fluorescence Flow Cytometry
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免疫動物 |
Rabbit Mono
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抗体クラス |
IgG
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クローン |
16F10
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交差種 |
Human
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Accession No.(Gene/Protein) |
P20023
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形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Positive WB detected in Raji whole cell lysate All lanes CD21 antibody at 0.55ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 155 KDa Observed band size: 155 KDa
IHC image of CY5710 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA005934A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Raji cells with CSB-RA005934A0HU at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing Raji cells stained with CSB-RA005934A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Western Blot Positive WB detected in Raji whole cell lysate All lanes CD21 antibody at 0.55ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 155 KDa Observed band size: 155 KDa
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メーカー |
品番 |
包装 |
CSB
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CSB-RA005934A0HU
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100 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
2週間程度
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保存温度 |
-20℃
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