別品名 |
RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry Immuno Fluorescence Immunoprecipitation
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免疫動物 |
Rabbit Mono
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抗体クラス |
IgG
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クローン |
4H12
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交差種 |
Human
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翻訳後修飾 |
リン酸化
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Accession No.(Gene/Protein) |
P31749
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形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not) All lanes Phospho-AKT1 antibody at 1.08ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60 KDa Observed band size: 60 KDa
IHC image of CSB-RA001553A473phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA001553A473phHU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells(treated with 100mM EGF for 20min) with CSB-RA001553A473phHU at 1:68,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating Phospho-AKT1 in 293 whole cell lysate treated with Calyculin A Lane 1: Rabbit control IgG(1ug)instead of CSB-RA001553A473phHU in 293 whole cell lysate treated with Calyculin A.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA001553A473phHU(3ug)+ 293 whole cell lysate treated with Calyculin A(1mg) Lane 3: 293 whole cell lysate treated with Calyculin A (20ug)
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Western Blot Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not) All lanes Phospho-AKT1 antibody at 1.08ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60 KDa Observed band size: 60 KDa
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メーカー |
品番 |
包装 |
CSB
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CSB-RA001553A473PHHU
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50 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
2週間程度
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保存温度 |
-20℃
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