Anti MSLN / Mesothelin  

Immunohistochemistry of Mouse anti-Mesothelin antibody. Tissue: tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Mesothelin antibody at 15 ﾂｵg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: Mesothelin as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry using the anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN.

Western blotting using the anti-mesothelin antibodies to detect mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng of CDC25-Fc. Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane. Primary antibody was used at 1ﾂｵg/ml followed by reaction with ALP goat anti-mouse IgG and BCIP/NBT substrate.