別品名 |
Flotillin-2;Epidermal surface antigen;ESA;Membrane component chromosome 17 surface marker 1;FLOT2;ESA1, M17S1
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Immunoprecipitation
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免疫動物 |
Rabbit Mono
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クローン |
GB-6
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交差種 |
Human Mouse Rat
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Accession No.(Gene/Protein) |
Q14254
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Gene Symbol |
FLOT2
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分子量 |
47064 MW
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形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot analysis of Flotillin-2 expression in (1) Hela cell lysate;(2) 293 cell lysate (M06107). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLOT2 monoclonal antibody (Catalog # M06107) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLOT2
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Western blot analysis of Flotillin-2 expression in (1) Hela cell lysate;(2) 293 cell lysate (M06107). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLOT2 monoclonal antibody (Catalog # M06107) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLOT2
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メーカー |
品番 |
包装 |
BBT
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M06107
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100 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
1週間程度
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保存温度 |
-20℃
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