別品名 |
Atypical chemokine receptor 3;C-X-C chemokine receptor type 7;CXC-R7;CXCR-7;Chemokine orphan receptor 1;G-protein coupled receptor 159;G-protein coupled receptor RDC1 homolog;RDC-1;ACKR3;CMKOR1, CXCR7, GPR159, RDC1
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot
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免疫動物 |
Rabbit Mono
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クローン |
FEI-1
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交差種 |
Human Mouse Rat
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Accession No.(Gene/Protein) |
P25106
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Gene Symbol |
ACKR3
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分子量 |
41493 MW
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形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot analysis of CXCR7 expression in K562 cell lysate (M02656). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACKR3 monoclonal antibody (Catalog # M02656) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACKR3
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Western blot analysis of CXCR7 expression in K562 cell lysate (M02656). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACKR3 monoclonal antibody (Catalog # M02656) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACKR3
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メーカー |
品番 |
包装 |
BBT
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M02656
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100 UL
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※表示価格について
当社在庫 |
なし
|
納期目安 |
1週間程度
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保存温度 |
-20℃
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