別品名 |
Homeobox protein Nkx-2.1;Homeobox protein NK-2 homolog A;Thyroid nuclear factor 1;Thyroid transcription factor 1;TTF-1;Thyroid-specific enhancer-binding protein;T/EBP;NKX2-1 ;NKX2A, TITF1, TTF1
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Immunohistochemistry Immuno Fluorescence
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免疫動物 |
Rabbit Mono
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クローン |
BIG-14
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交差種 |
Human Mouse Rat
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Accession No.(Gene/Protein) |
P43699
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Gene Symbol |
NKX2-1
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分子量 |
38596 MW
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形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemical analysis of paraffin-embedded human thyroid, using TTF1 Antibody(M01322-1)NKX2-1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NKX2-1 Antibody (M01322-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of TTF1 expression in 293 cell lysate (M01322-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKX2-1 monoclonal antibody (Catalog # M01322-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NKX2-1
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Immunohistochemical analysis of paraffin-embedded human thyroid, using TTF1 Antibody(M01322-1)NKX2-1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NKX2-1 Antibody (M01322-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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メーカー |
品番 |
包装 |
BBT
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M01322-1
|
100 UL
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
1週間程度
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保存温度 |
-20℃
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