別品名 |
G2/mitotic-specific cyclin-B1;CCNB1;CCNB
|
種由来 |
Human
|
標識物 |
Unlabeled
|
精製度 |
Affinity Purified
|
適用 |
Western Blot Immunohistochemistry Immuno Fluorescence
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免疫動物 |
Rabbit Mono
|
クローン |
EOG-3
|
交差種 |
Human Mouse
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Accession No.(Gene/Protein) |
P14635
|
Gene Symbol |
CCNB1
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分子量 |
48337 MW
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形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemical analysis of paraffin-embedded human colon cancer, using Cyclin B1 Antibody(M00745-2)CCNB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CCNB1 Antibody (M00745-2)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of Cyclin B1 expression in HeLa cell lysate (M00745-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 monoclonal antibody (Catalog # M00745-2) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using Cyclin B1 Antibody(M00745-2)CCNB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CCNB1 Antibody (M00745-2)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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メーカー |
品番 |
包装 |
BBT
|
M00745-2
|
100 UL
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
1週間程度
|
保存温度 |
-20℃
|
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