別品名 |
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1;3.2.2.6;2'-phospho-ADP-ribosyl cyclase;2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase;2.4.99.20;2'-phospho-cyclic-ADP-ribose transferase;ADP-ribosyl cyclase 1;ADPRC 1;Cyclic ADP-ribose hydrolase 1;cADPr hydrolase 1;T10;CD38;CD38
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種由来 |
Human
|
標識物 |
Unlabeled
|
精製度 |
Affinity Purified
|
適用 |
Western Blot
|
免疫動物 |
Rabbit Mono
|
クローン |
GAD-3
|
交差種 |
Human
|
Accession No.(Gene/Protein) |
P28907
|
Gene Symbol |
CD38
|
分子量 |
34328 MW
|
形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot analysis of CD38 expression in HeLa cell lysate (M00193). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD38 monoclonal antibody (Catalog # M00193) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD38
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|
Western blot analysis of CD38 expression in HeLa cell lysate (M00193). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD38 monoclonal antibody (Catalog # M00193) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD38
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|
メーカー |
品番 |
包装 |
BBT
|
M00193
|
100 UL
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
3週間程度
|
保存温度 |
-20℃
|
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