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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of SOX9 using anti-SOX9 antibody (M00177).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX9 antigen affinity purified monoclonal antibody (Catalog # M00177) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX9 at approximately 70 kDa. The expected band size for SOX9 is at 56 kDa.
Figure 2. IHC analysis of SOX9 using anti-SOX9 antibody (M00177).
SOX9 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SOX9 Antibody (M00177) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 3. IHC analysis of SOX9 using anti-SOX9 antibody (M00177).
SOX9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SOX9 Antibody (M00177) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Immunofluorescent analysis of SW480 cells, using SOX9 Antibody .
Figure 4. IHC analysis of SOX9 using anti-SOX9 antibody (M00177).
SOX9 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SOX9 Antibody (M00177) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 1. Western blot analysis of SOX9 using anti-SOX9 antibody (M00177).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX9 antigen affinity purified monoclonal antibody (Catalog # M00177) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX9 at approximately 70 kDa. The expected band size for SOX9 is at 56 kDa.
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| 別品名 |
Transcription factor SOX-9;SOX9;
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Immunohistochemistry
Immuno Fluorescence
Immunocytochemistry (cell)
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| 免疫動物 |
Rabbit Mono
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| クローン |
DAO-19
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| 抗体クラス |
IgG
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| Accession No.(Gene/Protein) |
P48436
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| Gene Symbol |
SOX9
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| 分子量 |
56137 MW
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| 概要 |
Boster Bio Anti-SOX9 Rabbit Monoclonal Antibody catalog # M00177. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
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| メーカー |
品番 |
包装 |
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BBT
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M00177
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100 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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