別品名 |
Tumor necrosis factor receptor superfamily member 6;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;FASLG receptor;CD95;FAS;APT1, FAS1, TNFRSF6;
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種由来 |
Human
|
標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Immunohistochemistry Immuno Fluorescence Immunocytochemistry (cell) Flow Cytometry
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免疫動物 |
Rabbit
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抗体クラス |
IgG
|
クローン |
IID-6
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交差種 |
Human
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Accession No.(Gene/Protein) |
P25445
|
Gene Symbol |
FAS
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分子量 |
37732 MW
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形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot analysis of Fas expression in Ramos cell lysate (M00055). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAS monoclonal antibody (Catalog # M00055) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAS
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|
Western blot analysis of Fas expression in Ramos cell lysate (M00055). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAS monoclonal antibody (Catalog # M00055) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAS
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メーカー |
品番 |
包装 |
BBT
|
M00055
|
100 UL
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
1週間程度
|
保存温度 |
-20℃
|
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