標識物 |
Horseradish Peroxidase
|
精製度 |
Affinity Purified
|
適用 |
Western Blot
|
免疫動物 |
Rabbit Mono
|
クローン |
DH-7
|
交差種 |
Human Mouse Rat
|
Gene Symbol |
GST-Tag
|
形状 |
液状
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot analysis of GST expression in GST recombinant protein, using GST tag Antibody(HRP conjugated) (HT0015). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST-Tag monoclonal antibody (Catalog # HT0015) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GST-Tag
|
|
Western blot analysis of GST expression in GST recombinant protein, using GST tag Antibody(HRP conjugated) (HT0015). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST-Tag monoclonal antibody (Catalog # HT0015) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GST-Tag
|
|
|
メーカー |
品番 |
包装 |
BBT
|
HT0015
|
100 UL
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
1週間程度
|
保存温度 |
-20℃
|
|