抗原部位 |
N-terminus
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種由来 |
Streptococcus pyogenes
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適用 |
Western Blot Immuno Fluorescence Immunoprecipitation
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免疫動物 |
Mouse
|
抗体クラス |
IgG1κ
|
交差種 |
Streptococcus pyogenes
|
GENE ID |
901176
|
Accession No.(Gene/Protein) |
Q99ZW2
|
Gene Symbol |
SPy_1046
|
形状 |
液状
|
参考文献 |
Yin K ,Han T, Liu G, Chen T, Wang Y, Yu AY, Liu Y. Sci Rep. 2015; 5: 14926. Liang X, Potter J, Kumar S, Zou Y, Quintanilla R, Sridharan M, Carte J, Chen W, Roark N, Ranganathan S, Ravinder N, Chesnut JD. J Biotechnol.2015 Aug 20;208:44-53. Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA. Cell.2015 Mar 12;160(6):1246-60. Aubrey BJ, Kelly GL, Kueh AJ, Brennan MS, O'Connor L, Milla L, Wilcox S, Tai L, Strasser A, Herold MJ. Cell Rep.2015 Mar 3;10(8):1422-32. Bailey SD, Desai K, Kron KJ, Mazrooei P, Sinnott-Armstrong NA, Treloar AE, Dowar M, Thu KL, Cescon DW, Silvester J, Yang SY, Wu X, Pezo RC, Haibe-Kains B, Mak TW, Bedard PL, Pugh TJ, Sallari RC, Lupien M. Nat Genet. 2016 Oct; 48(10): 1260?1266. van Sluis M, McStay B. Genes Dev.2015 Jun 1;29(11):1151-63. Liao HK, Gu Y, Diaz A, Marlett J, Takahashi Y, Li M, Suzuki K, Xu R, Hishida T, Chang CJ, Esteban CR, Young J, Izpisua Belmonte JC. Nat Commun.2015 Mar 10;6:6413. Byrne SM, Church GM. Curr ProtocStemCell Biol.2015;35:5A.8.1-22. Christopher A. de Solis, Anthony Ho, Roopashri Holehonnur, Jonathan E. Ploski. Front Mol Neurosci.2016 Aug 18;9:70.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunofluorescent staining of Hela cell line with antibody followed by incubation with an anti mouse secondary antibody (left). Nuclei were counter-stained with DAPI (right).
IP was performed on whole cell extracts (100 ug) from HEK293 cells transfected with a Flag-tagged Cas9. The immunoprecipitated proteins were subsequently analysed by Western Blot with the antibody. Lane 3 and 4 show the result of the IP; a negative IP control (IP on untransfected cells) and the input (15 ug) are shown in lane 2 and 1, respectively.
Western Blot (Cell lysate) analysis of (1) HeLa cells transfected with a flag-tagged Cas9, (2) 10 ng of recombinant Cas9 protein, and (3) 1 ng of recombinant Cas9 protein.
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Immunofluorescent staining of Hela cell line with antibody followed by incubation with an anti mouse secondary antibody (left). Nuclei were counter-stained with DAPI (right).
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メーカー |
品番 |
包装 |
ABV
|
MAB15818
|
50 UG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
1週間程度
|
保存温度 |
-70℃
|
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