別品名 |
Centriolar coiled-coil protein of 110 kDa; Centrosomal protein of 110 kDa; CP110; Cep110; CCP110; CEP110; CP110; KIAA0419
|
抗原部位 |
a.a.51-284
|
種由来 |
Human
|
標識物 |
Unlabeled
|
精製度 |
Affinity Purified
|
適用 |
Western Blot IHC paraffin embedding section Enzyme Linked Immunosorbent Assay
|
免疫動物 |
Rabbit
|
抗体クラス |
IgG
|
交差種 |
Human Mouse Rat
|
GENE ID |
9738
|
Accession No.(Gene/Protein) |
O43303
|
Gene Symbol |
CCP110
|
形状 |
凍結乾燥品
|
参考文献 |
1. Chen, Z., Indjeian, V. B., McManus, M., Wang, L., Dynlacht, B. D. CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells. Dev. Cell 3: 339-350, 2002. 2. Spektor A, Tsang WY, Khoo D, Dynlacht BD (August 2007). ''Cep97 and CP110 suppress a cilia assembly program''. Cell. 130 (4): 678-90.
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot analysis of CP110 using anti-CP110 antibody (A05058-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CP110 antigen affinity purified polyclonal antibody (Catalog # A05058-1) at 0.5 I?g/mL overnight at 4A°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CP110 at approximately 110KD. The expected band size for CP110 is at 113KD.
IHC analysis of CP110 using anti-CP110 antibody (A05058-1). CP110 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I?g/ml rabbit anti-CP110 Antibody (A05058-1) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of CP110 using anti-CP110 antibody (A05058-1). CP110 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I?g/ml rabbit anti-CP110 Antibody (A05058-1) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
|
|
Western blot analysis of CP110 using anti-CP110 antibody (A05058-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CP110 antigen affinity purified polyclonal antibody (Catalog # A05058-1) at 0.5 I?g/mL overnight at 4A°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CP110 at approximately 110KD. The expected band size for CP110 is at 113KD.
|
|
|
メーカー |
品番 |
包装 |
BBT
|
A05058-1
|
100 UG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
1週間程度
|
法規制 |
毒
|
保存温度 |
-20℃
|
|