Anti SAE2/UBA2  

データシート
別品名 SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2; UBLE1B; HRIHFB2115
抗原部位 a.a.449-564
種由来 Human
標識物 Unlabeled
精製度 Affinity Purified
適用 Western Blot
IHC paraffin embedding section
Immunocytochemistry (cell)
IHC frozen section
Flow Cytometry
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
免疫動物 Rabbit
抗体クラス IgG
交差種 Human
Mouse
Rat
GENE ID 10054
Gene Symbol UBA2
形状 凍結乾燥品
その他 [Uniprot ID]Q9UBT2
参考文献 1. ''Entrez Gene: ubiquitin-like modifier activating enzyme 2''.
2. Gong L, Li B, Millas S, Yeh ET (April 1999). ''Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex''. FEBS Lett. 448 (1): 185-9.
3. Desterro JM, Rodriguez MS, Kemp GD, Hay RT (April 1999). ''Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1''. J. Biol. Chem. 274 (15): 10618-24.

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Figure 1. Western blot analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (A03816-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: human SK-OV-3 whole cell lysates,Lane 6: human 22RV1 whole cell lysates,Lane 7: human A431 whole cell lysates,Lane 8: human COLO-320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE2/UBA2 antigen affinity purified polyclonal antibody (Catalog # A03816-2) at 0.5 I?g/mL overnight at 4A°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAE2/UBA2 at approximately 90KD. The expected band size for SAE2/UBA2 is at 71KD.

メーカー 品番 包装
BBT A03816-2 100 UG
希望販売価格 ¥59,000

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当社在庫 なし
納期目安 3週間程度
保存温度 -20℃

 

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メーカー情報
メーカー名 Boster Immunoleader
略号 BBT
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